conversion of IU/ml to mcg

From: megha goyal
Date: 19 January 2012 02:58

We are involved in R & D of recombinant filgrastim and the standard sample label mentions it as 30MiOU/ml i.e 300 mcg/ml. How can we determine the IU/ml as we know our protein is 300 mcg/ml. can anyone please guide me on the corelation.

 

regards,

 

megha

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From: Tim Gruene 

Hi Megha,

your email could hardly be more cryptic to me, and maybe you increase
the chance of getting help by explaining
- - what is R & D
- - what is MiOU?
- - what is mcg? (milli-centi-gram?)
(I understand ml, but I do not remember having met any of those other
units).

Cheers,
Tim

- --
- --
Dr Tim Gruene

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From: Narayanan Ramasubbu 

- - what is R&  D

We are involved in R&  D of recombinant filgrastim and the standard sample

Would these be
R&D - Research and Development?
mcg = microgram
IU/ml = International Units?/ml

I have no idea about MiOU? Probably some optical unit?

Subbu

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From: William Kennedy

Hey Narayanan

Not sure about about your spelling or capitalization but I am assuming the O is a mistake.  The, MiU is mega international units.

mcg is microgram ( when Greek symbol  can't be typed).

You will need to know the assay that measures activity for G-CSF (filgrastrim).  Then the standard reference for specific activity in units per mg or mcg of filgrastrim.  Conversion should follow.

So for Neuprogen, Filgrastim has a specific activity of 1.0 ± 0.6 x E08 U/mg (as measured by a cell mitogenesis assay), or 100 MU/mg, or 0.1 MU/mcg.

(NB R&D is Research and Development in Pharmaceutical acronym.)

Dexter Kennedy

Genentech, Inc.
iPawed from my iPad