on the electronic density of several maps

From: Dialing Pretty
Date: 13 January 2012 22:21

Dear All,

For the electronic density of LEU and Pro in the electronic density map, which is much stronger?

For the electronic density of LEU and Lys in the electronic density map, which is much stronger?

The reason I ask the above questions is I need to distinguish them in the electronic density map.

I am looking forward to getting your reply.

Fenghui

 

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From: Dale Tronrud

  It's not the strength of the electron density it's the shape that is
important.

Dale Tronrud

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From: Bernhard Rupp (Hofkristallrat a.D.)

You might want to look at some images of side chain electron density.

 

http://www.ruppweb.org/garland/gallery/Ch2/index_2.htm

 

BR

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From: Yuri Pompeu


Fenghui,
What is your resolution? If your having trouble distinguishing between pro and leu I am guessing it is
worse than 2.8 A.
You may not be able to model side chains confidently with lower resolution data. You may have to make a call
on wether or not to model side chains, and if your model is interesting enough to pursue even without side chains.