Thursday, 30 August 2012

Post-doctoral position in Lyon, France


From: Laurent Terradot
Date: 28 August 2012 12:36

Post-doctoral position in Structural Biology of Bacterial Pathogenesis at the IBCP (Lyon, France)

 

A FINOVI (http://www.finovi.org) funded postdoctoral position is available immediately in the group of Laurent Terradot at the Institut de Biologie et Chimie des Protéines (IBCP), Lyon, (http://www.ibcp.fr). We are interested in the structure/function of protein complexes involved in bacterial pathogenesis. The funded position will aim to solve the structures of type IV secretion system effectors and understand their mode(s) of action (see Tosi et al., Febs L, 2009; Jimenez-Soto et al., PloS Pathogens, 2009; Kaplan-Türköz et al., PNAS, 2012). The project is a collaboration between our laboratory and team of P. Doublet at the University of Lyon 1.

 

The IBCP is well equipped for Molecular Biology, Biochemistry, Bioinformatics and Structural Biology and belongs to a larger campus (UMS3444/US8, Lyon Biopole; Center for Infectiology), giving access to state-of-the-art core facilities, including crystallogenesis and biophysics platforms. The group has regular access to synchrotron beam time and is ideally located one hour from the European Synchrotron ESRF. Lyon is a vibrant, historical French city and is a world-excellence center in Infectiology.

 

We are looking for enthusiastic and self-motivated individuals with a strong background in X-ray crystallography, model building and refinement. Successful experience in protein purification for structural studies is required. Experience in protein-protein interactions (Biacore, ITC) and/or study of large complexes would be a plus. Working language of the laboratory is English.

 

Starting date: As soon as possible.

Salary is according to the CNRS french guidelines.

Please send a cover letter, your CV and reference letters as a single PDF file to: laurent.terradot@ibcp.fr

 

Address:

 

Laurent Terradot

 

Institut de Biologie et Chimie des Protéines

ATIP-CNRS Group Structural Biology of Bacterial Macromolecular Complexes

UMR 5086 CNRS Université de Lyon

7, passage du Vercors

69367 Lyon cedex 07 FRANCE

Symposium in Membrane Protein Crystallisation - UK


From: Dr. Isabel De Moraes
Date: 10 August 2012 17:31

Dear All,

The Membrane Protein Laboratory (MPL) at Diamond Light Source is organising a Symposium in Membrane Protein Crystallisation on the 27th of September at the Research Complex at Harwell - UK

The registration is now open:
http://www.rc-harwell.ac.uk/symposium-in-membrane-protein-crystallization

There are a maximum of 75 places and the registration will be closed once all the places are taken.
Program
                Symposium in Membrane Protein Crystallization
9:45 - 10:15    arrival/registration
10:15 - 10:30   welcome
Session 1       Chair: Prof. Steve Mathews - Imperial College London
10:30 -11:00    Introduction to Membrane Protein Crystallization
Isabel Moraes - Diamond - MPL
11:00 -11:30    Stabilising Membrane Protein Samples for Crystallization
Paul Thaw and Jeanette Hobbs - Molecular Dimensions
11:30 - 12:00   lipidic Cubic Phase Technologies for Membrane Protein Structural Studies
Vadim Cherezov - The Scripps Research Institute - USA
12:00 - 12:30   Membrane Protein Nano and Macro Crystallization using Lipidic Sponge Phases
Linda Johansson - University of Gothenburg - Sweden
12:30 - 13:30   lunch
Session 2       Chair: Prof. Thomas Sorensen - Diamond Light Source
13:30 - 14:00   The Use of Bicelles in Membrane Protein Crystallization
Jeff Abramson - University of California, Los Angeles - USA
14:00 - 14:30   Current Trends in Alpha Helical Membrane Protein crystallization - The Rationale Behind MemGold, MemGold2 and MemAdvantage Screens
Simon Newstead - University of Oxford
14:30 - 15:00   Advances in Automated Imaging and Liquid Handling to Support Membrane Protein Research
Olivia Sleator - Rigaku
15:00 - 15:30   Crystallizing GPCRs for Drug Discovery
João Dias - Heptares
15:30 - 16:00   Break
session 3       Chair: Dr Alex Cameron - Imperial College London
16:00 - 16:30   Imaging Protein Crystals with Ultrafast Lasers
Ellen Gualtieri - Formulatrix
16:30 - 17:00   Exploiting Microfocused X-rays for Challenging Problems in MX
Robin Owen - Diamond Light Source
17:00 - 17:30   BLEND - The systematic Scaling and Merging of Multiple Datasets in Macromolecular crystallography
James Foadi - Diamond - MPL
17:30   Closing remarks



Sponsors
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 [http://www.diamond.ac.uk/Home/Events/Symposium-in-Membrane-Protein-Crystallization/mainColumnParagraphs/01/text_files/file/dibanner.png] <http://www.douglas.co.uk/>   [http://www.rigaku.com/sites/default/files/Rigaku_0.jpg] <http://www.rigaku.com/>
 [http://www.rc-harwell.ac.uk/assets/site_files/img/header_logo.gif] <http://www.rc-harwell.ac.uk/>

[http://www.diamond.ac.uk/Home/Events/Symposium-in-Membrane-Protein-Crystallization/mainColumnParagraphs/01/text_files/file0/WellcomeTrust.png]<http://www.wellcome.ac.uk/>




[http://www.diamond.ac.uk/Home/Events/Symposium-in-Membrane-Protein-Crystallization/mainColumnParagraphs/01/text_files/file1/Image1.png]<http://diamond.ac.uk/>
         [http://www3.imperial.ac.uk/2007templates/images/logo_imperial_college_london.png] <http://www3.imperial.ac.uk/>

We would be delighted to meet you at the symposium.


With best Regards,
Isabel Moraes

-----------------------------------------------------------------------------------------
Dr. Isabel De Moraes, MRSC

Membrane Protein Laboratory Facility Co-ordinator
Membrane Protein Laboratory
Diamond Light Source Ltd,
Chilton, Didcot, Oxfordshire,
OX11 ODE, UK

Postdoctoral postion


From: Faiz Mohammed
Date: 30 August 2012 19:54


Hi,

A post-doctoral opening in protein crystallography in the laboratory of Prof. Chris Dealwis at the Cleveland Center for Membrane and Structural biology. The project involves structure determination of protein-protein complexes and protein drug interactions. We are seeking for an individual that has a Ph.D in protein crystallography. Some basic biochemistry skills involving protein expression and purification will be extremely valuable. Candidates should send their CV directly to Chris Dealwis 
http://pharmacology.case.edu/department/Faculty/Primary/Pages/dealwis.aspx
http://www.ncbi.nlm.nih.gov/pubmed?term=Dealwis%20Chris

Best,
Faiz, Ph.D.


Saturday, 25 August 2012

Postdoc positions in X-ray crystallography or electron microscopy at Imperial College London

From: P Freemont
Date: 20 August 2012 13:40


Research Associates: London, United Kingdom
Imperial College London

Salary: £32,100 - £40,720 per annum

Two Research Associate posts are available in the the research group of Professor Paul Freemont (www.msf.bio.ac.uk) in the in the Centre for Structural Biology, at the South Kensington Campus of Imperial College London. Funded by Cancer Research UK and Medical Research Council  you will join a group of multi-disciplinary colleagues and collaborators on two unrelated projects. Project one (NS 2012 168 IL) in collaboration with  Professor Xiaodong Zhang is aimed at understanding the  molecular mechanism and functions of the mammalian AAA ATPase p97 whilst Project two (NS 2012 176 IL) in collaboration with Professor Alain Filloux, will investigate the structure and function of the Type VI secretion system in Pseudomonas aeruginosa.

You must hold a PhD in a structural biology or biochemistry discipline or a related discipline, or an equivalent level of professional qualifications and experience. Research experience in a structural biological laboratory environment (covering  X-ray crystallography, and/or electron microscopy single particle analysis), and knowledge of protein biochemistry are essential. 

For informal enquires please contact Professor Paul Freemont 

Our preferred method of application is online via our website http://www3.imperial.ac.uk/employment (please select "Job Search" then enter the job title or vacancy reference – number ( in bold above) including spaces into "Keywords").  Please complete and upload an application form as directed.


Closing date for NS 2012 168 IL : 7 September 2012 (Midnight BST)

Closing date for NS 2012 176 IL : 17 September 2012 (Midnight BST)

***********************************************
Professor Paul Freemont
Head of Division of Molecular Biosciences
Faculty of Natural Sciences
Imperial College London
South Kensington Campus
London SW7 2AZ, UK
www.imperial.ac.uk/molecularbiosciences
Group: www.msf.bio.ic.ac.uk
Co-director of EPSRC Centre for Synthetic Biology and Innovation at Imperial
***********************************************











Wednesday, 22 August 2012

Senior/Principal Scientist – Protein Crystallography


From: Seungil Han
Date: 20 August 2012 20:48

The successful candidate for this position will join Groton Structural Biology and Biophysics (SB&B), a multi-disciplinary group with well-established expertise in molecular biology, protein biochemistry, biological mass spectrometry, NMR, biophysics, crystallography & computational sciences.  Our mission is to provide integrated molecular insights into drug target mechanism and target-ligand interactions to impact discovery efforts across important therapeutic areas, such as Cardiovascular, Metabolic & Endocrine Diseases, Neuroscience, Immunology & Autoimmune Diseases, Inflammation & Remodeling, Orphan & Genetic Diseases Pain and Oncology.

 

As a Senior/Principal Scientist - Protein Crystallography, you will be able to use your creativity, energy and expertise to contribute to cutting edge science and drug discovery through structural studies of a human integral membrane protein, building upon the exciting advances already made by the group. You will drive the progression of the project through protein purification and detergent optimization, crystallization and structural analysis.  You may have the opportunity to explore necessary biophysical techniques to characterize your proteins or solve specific scientific problems, and learn new techniques from experts in these areas.  You may also have the opportunity to develop a broad understanding of other disciplines involved in drug discovery.



Interested individuals should submit a CV, statement of research  and contact information for three references at

https://jobs.pfizer.com/psc/recruit/EMPLOYEE/HRMS/c/LSYS_DEVELOPMENT.Z_PFIZER_JOBS.GBL?country=USA&languagecd=ENG

The job ID: ID 970242


Inquiries regarding the position can be directed to Dr. Xiaomin Chen

Monday, 20 August 2012

PhD position available

From: Andy-Mark Thunnissen
Date: 20 August 2012 11:57


A 4-year PhD position is offered at the Biomolecular Sciences and Biotechnology Institute, University of Groningen, the Netherlands.

Starting date: position is open.

Description

The successful PhD candidate will determine X-ray structures of novel cytochrome P450 enzymes, to understand the structural basis of their selectivity and catalytic properties, and to use this insight for developing improved P450 variants. Furthermore, the successful candidate will participate in the activities of the FP7 Marie Curie Initial Training Network called P4FIFTY (http://www.p4fifty.eu), including research collaborations with 8 academic and 2 industrial partners.

Qualifications

To be eligible, applicants must hold an MSc degree, or comparable, in biophysical chemistry, biochemistry or similar. Experience with protein production/purification, basic molecular biology, X-ray crystallography and biochemical/biophysical characterization of proteins is highly desirable. Applicants should also have good knowledge of English, both written and oral, as the successful candidate will be asked to submit reports and the doctoral thesis in English. The position is offered in the context of a Marie Curie Initial Training Network, and transnational (European) mobility is a key element of eligibility. As such, applications will only be accepted from EU candidates who have spent less than 12 months in the Netherlands within the last three years. Candidates' eligibility for the post is determined by Marie Curie terms and conditions.

Application information

Further details of the post and application procedure are available at: http://www.rug.nl/corporate/vacatures/jobOpportunitiesRUG

(look for job title: PhD position Crystallography of P450s)

Applicants should send:

  • Their Curriculum Vitae
  • A covering letter explaining their motivation for applying and any previous research experience or project work.
  • Two names and contact information for references

Please note that, if interested, you will need to apply online through the link above.

For more information you can contact Dr. Andy-Mark W. H. Thunnissen, 




Mac or PC?

From: Lee, Ting Wai
Date: 9 August 2012 20:52


Hi, everyone.
 
May I ask a very general question? I am going to buy a laptop. I am going to do a lot of structural biology work on it using programs such as CCP4, Phenix, Coot and Pymol. Mac or PC, which is better? I have never installed this kind of programs and done structural biology work on laptops except using Pymol. Will these programs cause any problems when they are run on laptops? I mean, will they slow down very much or even freeze the laptops? Can the programs finish the jobs at an OK speed? I mean, maybe not as fast as desktops, but not taking too long like days or weeks.
 
Sorry if this question is stupid. I hope to have your advice. Thank you.
 
HS
 

----------
From: Bosch, Juergen



On Aug 9, 2012, at 3:52 PM, Lee, Ting Wai wrote:

I mean, maybe not as fast as desktops, but not taking too long like days or weeks.

Sorry that time is over when you could get a cup of coffee while refinement of a structure was running.
Any system will work as long as you have sufficient RAM, if you invest into SSD you'll get spoiled very quickly.
Modern laptops are as powerful as 3 year old Desktop machines (I'm comparing Macbook Air 2012 versus MacPro 2009, if you run Geekbench on them you get tears how slow the 16 Core cluster is compared to the 4 core MacBook Air.

Jürgen


 
Sorry if this question is stupid. I hope to have your advice. Thank you.
 
HS
 




----------
From: Nat Echols


On Thu, Aug 9, 2012 at 12:52 PM, Lee, Ting Wai <> wrote:
> May I ask a very general question? I am going to buy a laptop. I am going to
> do a lot of structural biology work on it using programs such as CCP4,
> Phenix, Coot and Pymol. Mac or PC, which is better?

See this morning's thread.  Short answer: either works, just avoid Windows.

> I have never installed
> this kind of programs and done structural biology work on laptops except
> using Pymol. Will these programs cause any problems when they are run on
> laptops? I mean, will they slow down very much or even freeze the laptops?
> Can the programs finish the jobs at an OK speed? I mean, maybe not as fast
> as desktops, but not taking too long like days or weeks.

It depends on how big the structures you work with are, and what
you're trying to run.  I have a MacBook Air and it is quite adequate
for crystallography, but I've only worked with small and/or
low-resolution structures where there's no danger of exceeding the 4GB
memory limit.  (Of course, one can buy far more powerful laptops, but
the price goes up steeply.)  The important thing is *not* to buy the
cheapest PC laptop you can find, because the really low-end hardware
probably won't work very well.

-Nat

----------
From: HS


Thank you for your information. Actually I'm using my friend's old email account. I forgot to change the name. My name is HS.
 
HS