Saturday, 3 March 2012

before doing an elisa or buying a dye for affinity measurement....


From: Paul Kraft
Date: 9 February 2012 21:23


assuming your protein has a Y or a W...before buying a dye or trying an elisa, it would be cheapest and easiest to check for Trp or Tyr fluorescence changes upon adding the inhibitor. If the structure or a structural homolog is known of one of the proteins and you know the interface, a simple PCR rxn to generate a W mutant would be easy too. Dye detection methods  can be problematic at times, and elisa's have there own problems...
 
Dr. Paul Kraft
Structural Biologist



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