Wednesday, 2 November 2011

Phenix building residues not found in input sequence file

From: Allan Pang
Date: 18 October 2011 16:38

Hi everyone,

Apparently my Phenix (1.6.3-473) is building in extra residues in the C-terminal region, which is not found in my input sequence file.

It's trying to build a model which is longer than I expected. Phenix building 87 extra amino acids. Any clue what's wrong?

I made sure that these amino acids are not residues found in the N-terminal region or anywhere else in the protein.

Allan

--
Allan Pang

PhD Student

G35 Joseph Priestley Building
Queen Mary University of London
London
E1 4NS



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From: Vellieux Frederic
Could you have an unexpected "small subunit" (or some other situation) giving additional density that Phenix is building into ? In other words, how sure are you of what is present in the crystal ? This is because I remember the enzyme methanol dehydrogenase where there was a small subunit which had not been evidenced for years...

Fred.


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