From: Reginald McNulty
Date: 27 October 2011 00:08
----------
From: Jürgen Bosch
----------
From: Morten Groftehauge
Lots of systems go to 4C. Even a cheapo one like the Applied Biosystems StepOnePlus.
Date: 27 October 2011 00:08
We are trying to determine Tm value of rather unstable proteins with a Tm in
the mid 20 C range using DSF/thermofluor. Our Stratagene thermocycler has a
low temp limit of 25 C (Peltier). I called the company and they said it's a
'hardware limit' that cannot be changed.
1) Has anyone been able to 'hotwire' the MX3000/3005 to go below this limit?
2) Are there other thermofluor machines that allow lower starting
temperatures (say 4, 10 or 15 C)?
the mid 20 C range using DSF/thermofluor. Our Stratagene thermocycler has a
low temp limit of 25 C (Peltier). I called the company and they said it's a
'hardware limit' that cannot be changed.
1) Has anyone been able to 'hotwire' the MX3000/3005 to go below this limit?
2) Are there other thermofluor machines that allow lower starting
temperatures (say 4, 10 or 15 C)?
Best regards,
-Reggie
----------
From: Jürgen Bosch
CD spec with Pelletier is an option too
Jürgen
......................
......................
Jürgen Bosch
Johns Hopkins Bloomberg School of Public Health
Department of Biochemistry & Molecular Biology
Johns Hopkins Malaria Research Institute
615 North Wolfe Street, W8708
Baltimore, MD 21205
----------
From: Morten Groftehauge
Lots of systems go to 4C. Even a cheapo one like the Applied Biosystems StepOnePlus.
-Morten Groftehauge
No comments:
Post a Comment