about heavy atom derivatization

From: Shanti Pal Gangwar
Date: 30 March 2012 14:34
Dear all

I am beginner in crystallography.We have collected a native data of a given protein at 2.2A resolution but are unable t solve by MR
 therefore we collected Hg, and Pt derivative after  soaking the crystals. I don't know how to process this heavy atom derivative data
and find the anomalous signal if heavy atom is there?

thanks in advance for help and suggestions..


regards

Shanti Pal Gangwar



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From: Ed Pozharski
http://www.ccp4.ac.uk/dist/examples/tutorial/html/heavy-tutorial-mir.html

seems relevant

--
Edwin Pozharski, PhD, 

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From: Francis E Reyes
To add to Ed's comments:


Find someone in the immediate department/area to walk you through your first structure.


But to answer your question:

What about the MR didn't work?

Use your MR model to find your heavy atoms. They can be found using anomalous data  difference fouriers (or even better log likelihood gradient maps) using data  as low as 6-8A, and, in my case, using a model that was about 50% (mass) of the ASU with the placement of that 50% to be about 80-90% correct.


F

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Francis E. Reyes M.Sc.