Wednesday, 7 September 2011

Detergents to eliminate non specific aggregations


From: 商元 <shangyuan
Date: 27 August 2011 12:55

Hi, everyone,
   I can get my protein complex but there are some non-specific aggregation from the NMR spectra, and chaps can improve it.
So, besides chaps, is there any other detergents to be used during crystal screening? All suggestions are welcome.

Thanks&Regards,
Yuan

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From: Michael Thompson


Hampton makes a "Detergent Screen" that works the same way as the Additive Screen.

http://hamptonresearch.com/product_detail.aspx?cid=1&sid=39&pid=31

It has 96 unique detergent reagents for crystal screening.

It is a bit expensive, but if you don't want to purchase the whole kit you could just download the formulation table and then you will have a nice long list of possibilities.

Mike
--
Michael C. Thompson

Graduate Student

Biochemistry & Molecular Biology Division

Department of Chemistry & Biochemistry

University of California, Los Angeles


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