real dimer vs crystal packing

From: Qiang Chen
Date: 20 February 2012 21:21


Thanks, Artem!
PDE2A also uses three domains to form a homodimmer. However, without the
catalytic domain, the GAF B domain swings out. This is an excellent
example for the restrictions set by the multidomain context.

Anyone knows other examples?
Thanks a lot!

> PDE2 full length versus domain only structures, I think.
>
> Artem
>
> On Thu, Jan 12, 2012 at 10:03 AM, Qiang Chen >wrote:
>
>> Hi,
>>
>> I'm working on a multi-domain protein which uses three domains to form
>> homodimer, and the full-length structure is available. We have solved
>> the
>> structure of one binding domain alone and found its homo-binding mode is
>> totally different from that of the full-length protein.
>>
>> Do you know examples or papers discussing the similar subjects (crystal
>> packing shows false binding mode)?
>>
>> Thanks a lot!
>>
>> Qiang
>>
>>
>>
>>