recombinant sources of N-glycanase

From: Pascal Egea
Date: 11 March 2012 20:02

Hi All,

We are trying to deal with a eukaryotic membrane protein that crystallizes but, as usual, diffracts poorly so far. As it is heavily glycosylated, we are considering enzymatic deglycosylation.

Does anyone know about a recombinant source of N-glycanase ; we would like to prepare it ourselves because the commercial sources are apparently too expensive.

Thanks in advance.

--
Pascal F. Egea, PhD