effect of Izit dye within the crystal structure

From: adam andres
Date: 11 November 2011 04:34

Hi crystallographers

Has anyone actually collected data on a crystal that has been treated with Izit dye? If so did you see any structural effects or know any structure that present this dye? 
 
Cheers

 Adam Campos

----------
From: Bosch, Juergen

I wouldn't be surprised if it is methylene blue.

Jürgen

......................

Jürgen Bosch

Johns Hopkins University

Bloomberg School of Public Health

Department of Biochemistry & Molecular Biology

Johns Hopkins Malaria Research Institute

http://web.mac.com/bosch_lab/

----------
From: Hargreaves, David

I've collected data from crystal soaked with brightly coloured ligands which partition into the solvent channels. Although the crystal becomes highly coloured (mopping up most of the colour from the drop) the structures remained resolutely empty. I am intrigued by this observation and wonder how mutating the residues that constitute the solvent channels would affect soakbility and other crystal properties.

 

David Hargreaves

Associate Principal Scientist

_____________________________________________________________________

AstraZeneca

David.Hargreaves @astrazeneca.com

 

----------
From: Artem Evdokimov

Typically provided that the dye does not kill the crystal, there is no effect on structure, at least nothing I ever saw. The effect is similar to sizing column.
Artem

----------
From: Savvas Savvides

Dear Adam and colleagues

The enzyme human glutathione reductase (hGR) binds dyes such as safranin and xanthene, which are analogous to methylene blue (aka IZIT) with respect to their heterocyclic ring structures, in a cavity deep at the dimer interface. Safranin and xanthene are also inhibitors of hGR and crystal structures of complexes of GR with safranin and xanthene have been reported:

Karplus et al. 1989. Eur J Biochem. 178(3):693-703.

Savvides & Karplus 1996. Journal of Biological Chemistry 271(14):8101-7.

Given the general resemblance of these compounds to methylene blue and just to satisfy my curiosity at the time, I soaked methylene-blue into crystals of GR and collected good data to 2 angs. I was basically able to confirm that methylene blue bound specifically in the cavity at the dimer interface just like the other two compounds. However, this work has remained unreported.

best regards

Savvas