improve protein-DNA complex crystal

From: dengzq1987
Date: 21 November 2011 07:36

Hi all,

 

I got a protein-DNA complex crystal,by running SDS-PAGE and nativePAGE,i prove that the crystal contain both

protein and DNA.BUT it does not diffract. does anyone have suggestion to improve the diffraction ability ?

 

 

Best wishes,

  

deng

----------
From: zq deng

my DNA  contains a 9-1-9 palindromic sequence,i tried different blunt end DNA,and got two kind of crystal  with different lenght DNA.both don't diffract.

 

2011/11/21 Michael Murphy <pn1924@gmail.com>

Deng, could you tell us a bit more about the DNA that you used? I think you may want to try to optimize the DNA that you are using. Maybe try either a couple base pair longer/shorter piece of DNA? or try switching from blunt ended DNA to a one or two base pair overhang piece of DNA? (very often it is contacts between the ends of the pieces of DNA that drive crystallization)