Protein-Protein Complex Screening

From: Yi-Liang Liu
Date: 17 November 2011 20:03


Hi CCP4ers,

I know it is not quiet related to CCP4. I am now working on a protein-protein complex system. I am wondering which kits I should try in a higher priority? I appreciate everyone's suggestions, and maybe there are some papers I can read first.

Lucas
----------
From: Ho Leung Ng


There is at least one paper describing the success of PEG precipitants
for complexes, but I can't find the reference right now.


Ho


Ho Leung Ng
University of Hawaii at Manoa
Assistant Professor, Department of Chemistry

----------
From: Edward A. Berry 

Is this it?
http://scripts.iucr.org/cgi-bin/paper?S0021889802013973
J. Appl. Cryst. (2002). 35, 674-676    [ doi:10.1107/S0021889802013973 ]
Crystallization of protein-protein complexes
S. Radaev and P. D. Sun
Abstract: Crystallizing protein-protein complexes remains a rate-limiting step in their structure characterization. Crystallization conditions for the known protein-protein complexes have been surveyed in both the Protein Data Bank and the BMCD database. Compared with non-complexed proteins, crystallization conditions for protein-protein complexes are less diverse and heavily favor (71% versus 27%) polyethylene glycols (PEG) rather than ammonium sulfate or other high-salt crystallization conditions. The results suggest that the stability of protein complexes limits their available crystallization configuration space. Based on the survey, a set of sparse-matrix screen conditions was designed.

----------
From: Ed Pozharski

1. Radaev S, Li S, Sun PD. A survey of protein-protein complex
crystallizations. Acta Crystallogr. D Biol. Crystallogr. 2006 Jun;62(Pt
6):605-612.[cited 2011 Nov 18 ]

2. Radaev S, Sun PD. Crystallization of protein–protein complexes.
Journal of Applied Crystallography 2002 Nov;35:674-676.[cited 2011 Nov
18 ]


Nextal sells the Protein Complex Suite.  Based on the above reviews, it
may be a good idea to start with something heavy on PEG.

Good luck,

Ed.

----------
From: Zhou, Tongqing (NIH/VRC) [E]


I would recommend Qiagen's ProComplex and JCSG+, and Emerald's Precipitant Synergy.