Expression of a Selenomethionine Variant in E. coli

From: Yibin Lin
Date: 12 December 2011 21:56

Hi List,

I want to preprare sel-met labeling protein. Could someone can tell me
if it is necessary to add DTT or BME to medium, which contains
sel-met, when growing cells?

Thanks in advance,


Yibin Lin

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From: Antony Oliver


Generally no, only in the subsequent protein purification steps.

Tony.

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