From: Cory Brooks
Date: 16 February 2012 22:53
Date: 16 February 2012 22:53
Hello all;
I am wondering if anyone has any experience getting a ARI Gryphon robot to
pick up protein in a bicelle solution?
The nano needle on our robot does not seem to want to suck the sample up
in a variety of liquid class settings.
Any suggestions out there on if this can be made to work?
Cheers,
Cory
Cory Brooks,Ph.D.
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From: Art Robbins
Hello Cory,
Unfortunately your Crystal Gryphon was not designed to work with all bicelle solutions.
However, your Crystal Gryphon is easily upgradeable with our new LCP attachment.
This will allow you to do LCP, Bicelle and Sponge Phase experiments.
If you have any further questions about the Gryphon please contact David
Terrell or Dave Wright at our office (888 658 5300).
Regards,
Art Robbins
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From: Pius Padayatti
there is one nice JOVE video and article from Jeff Abramson lab
which was neat showing using mosquito to set up bicelle.
No offense to Art people
J Vis Exp. 2012 Jan 9;(59). pii: 3383. doi: 10.3791/3383.
High-throughput Crystallization of Membrane Proteins Using the Lipidic
Bicelle Method.
Ujwal R, Abramson J.
link video
http://www.jove.com/video/3383/high-throughput-crystallization-of-membrane-proteins-using-the-lipidic-bicelle-method
padayatti
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From: Poul Nissen
Sorry for self promotion but you might also consider the HILIDE method which is very similar to bicelle and sponge, only having fully solubilized protein:lipid:detergent complexes as input sample and therefore being fully compatible with regular liquid handling robotics.
See Gourdon P et al 2011, Crystal Growth & Design 11, 2098-2106 for the method, and Sonntag Y et al 2011, Nature Comm 2, 304 for a direct look at the bilayers formed in the crystal
Poul
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