endotoxin removal: Triton X-114 dissociates protein oligomers?

From: Jerry McCully
Date: 14 March 2012 02:26

Dear ALL;

         Thanks a lot for all the instructive suggestions. As my first trial, I tried 0.1%Triton X-114 plus Ni-column binding buffer.

          However, the oligomer of my protein got dissociated in to monomers as indicated by gel-filtration.

         Does anyone know how to rescue the oligomer?   Simply lower the concentration of Triton X-114 to 0.02-0.05%?

         Thanks again,

Jerry