From: 商元 <shangyuan
Date: 27 August 2011 12:55
Hi, everyone,
I can get my protein complex but there are some non-specific aggregation from the NMR spectra, and chaps can improve it.
So, besides chaps, is there any other detergents to be used during crystal screening? All suggestions are welcome.
Thanks&Regards,
Yuan
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From: Michael Thompson
Hampton makes a "Detergent Screen" that works the same way as the Additive Screen.
http://hamptonresearch.com/product_detail.aspx?cid=1&sid=39&pid=31
It has 96 unique detergent reagents for crystal screening.
It is a bit expensive, but if you don't want to purchase the whole kit you could just download the formulation table and then you will have a nice long list of possibilities.
Mike
--
Michael C. Thompson
Graduate Student
Biochemistry & Molecular Biology Division
Department of Chemistry & Biochemistry
University of California, Los Angeles
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