But...what's on my tyrosine?

From: Jacob Keller

Date: 28 October 2011 02:51



Here is a real bona fide "name that blob." Protein was expressed in
ecoli, nothing funny to it, native protein. Crystals frozen in NaCl,
HEPES, DTT, PEG3350, KBr, NaCO3, NaMES, Sucrose. Maybe somehow a
conjugated CO2 molecule from the bicarb (there seems to be a clear CO2
nearby.) Or perhaps a radiation-damage species?

JPK

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Jacob Pearson Keller
Northwestern University
Medical Scientist Training Program

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From: Ivan Shabalin


I would just model it with one water. Especially if resolution is worse than 1.8 (I dont think you have better based on the map)
Only if resolution is high and R-factors are low I would worry about this peak.
Regards,
Ivan