From: Dr. STEPHEN SIN-YIN, CHUI
Date: 2011/10/31
Dear all,
I know somebody had asked such question before, can anyone of you can remind me
the way of converting Bruker in-house data (*.sfrm) to the format readable by
MOSFLM in CCP4i by using the GUI version of FrmUltility.exe?
When I input the sfrm, and choose the tab "MSOFLM", i did not change any
setting, and it asked for P4P or SPIN, then I choose "LINEAR". Also for the tab
_IX, I also select "LINEAR". Finally I pressed "Unwarp, Flip & Convert" button
to convert it, the following message come:
FATAL dimensions of correction frame(s)different than data frame
Spatial table is 0x0, while data frame is 1024x1024
Please kindly advise, many thanks
stephen
--
Dr. Stephen Sin-Yin Chui (徐先賢)
Assistant Professor,
Department of Chemistry,
The University of Hong Kong, Pokfulam Road,
Hong Kong SAR, China.
----------
From: Owen Johnson
https://www.jiscmail.ac.uk/cgi-bin/webadmin?A2=CCP4BB;7dda3e7c.0705
----------
From: Edward Lowe
Dear Stephen,
Unfortunately you will find that the MOSFLM tab is not very helpful. The
"Flip" part of the operation generates images that are not readable by
MOSFLM - I think it may have been needed for older versions of the Bruker
image format.
Fortunately, it is perfectly possible to generate images suitable for
MOSFLM using FrmUtility.exe although it is a two-step process.
- Optionally, copy the images you wish to convert into a new folder. I
like to do this since I like to keep an unaltered copy of my raw data and
this is easier than recovering from the backup images made during
unwarping.
- Now you will need to unwarp the images. Select the Unwarp frame tab and
a suitable project folder and image range. You will need a spatial
correction file (this will be a p4p file if you are using Proteum
software). It must be in the same directory as you images for
FrmUtility.exe to find it. Normally it will be created in a sub-directory
called "work" so you will need to copy it. If you cannot find this, any
p4p file generated by the same detector should work.
- Finally You need to convert the image format to one compatible with
MOSFLM. Select the Convert format tab, and use your unwarped images as
input. Select the options "Bruker/BIS format" and "2-byte (MOSFLM)" and
hit convert.
Hope that helps,
Ed.
--
Dr. E.D. Lowe
Department of Biochemistry
University of Oxford
South Parks Road
Oxford, UK
OX1 3QU
Date: 2011/10/31
Dear all,
I know somebody had asked such question before, can anyone of you can remind me
the way of converting Bruker in-house data (*.sfrm) to the format readable by
MOSFLM in CCP4i by using the GUI version of FrmUltility.exe?
When I input the sfrm, and choose the tab "MSOFLM", i did not change any
setting, and it asked for P4P or SPIN, then I choose "LINEAR". Also for the tab
_IX, I also select "LINEAR". Finally I pressed "Unwarp, Flip & Convert" button
to convert it, the following message come:
FATAL dimensions of correction frame(s)different than data frame
Spatial table is 0x0, while data frame is 1024x1024
Please kindly advise, many thanks
stephen
--
Dr. Stephen Sin-Yin Chui (徐先賢)
Assistant Professor,
Department of Chemistry,
The University of Hong Kong, Pokfulam Road,
Hong Kong SAR, China.
----------
From: Owen Johnson
https://www.jiscmail.ac.uk/cgi-bin/webadmin?A2=CCP4BB;7dda3e7c.0705
----------
From: Edward Lowe
Dear Stephen,
Unfortunately you will find that the MOSFLM tab is not very helpful. The
"Flip" part of the operation generates images that are not readable by
MOSFLM - I think it may have been needed for older versions of the Bruker
image format.
Fortunately, it is perfectly possible to generate images suitable for
MOSFLM using FrmUtility.exe although it is a two-step process.
- Optionally, copy the images you wish to convert into a new folder. I
like to do this since I like to keep an unaltered copy of my raw data and
this is easier than recovering from the backup images made during
unwarping.
- Now you will need to unwarp the images. Select the Unwarp frame tab and
a suitable project folder and image range. You will need a spatial
correction file (this will be a p4p file if you are using Proteum
software). It must be in the same directory as you images for
FrmUtility.exe to find it. Normally it will be created in a sub-directory
called "work" so you will need to copy it. If you cannot find this, any
p4p file generated by the same detector should work.
- Finally You need to convert the image format to one compatible with
MOSFLM. Select the Convert format tab, and use your unwarped images as
input. Select the options "Bruker/BIS format" and "2-byte (MOSFLM)" and
hit convert.
Hope that helps,
Ed.
--
Dr. E.D. Lowe
Department of Biochemistry
University of Oxford
South Parks Road
Oxford, UK
OX1 3QU
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